Refine your search
Collections
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z All
Chandra Shekar, M.
- Molecular Characterization of Meghalaya Local Pigs (Niang Megha) using Microsatellite Markers
Abstract Views :414 |
PDF Views:0
Authors
Affiliations
1 Department of Animal Genetics & Breeding, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati–781022, Assam, IN
1 Department of Animal Genetics & Breeding, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati–781022, Assam, IN
Source
Indian Journal of Science and Technology, Vol 6, No 10 (2013), Pagination: 5302-5306Abstract
Molecular characterization using 21 microsatellite markers recommended by Food and Agricultural Organization of United Nations (FAO) for Swine revealed less genetic diversity in Meghalaya Local pig (Niang Megha). The loci used for investigation are informative. The number of observed alleles (Na) detected ranged from 2 to 6, with an overall mean of 3.9±1.30. A total of 82 alleles were observed across all the loci. The effective number of alleles (Ne) ranged from 1.145 to 4.2169 with a mean of 2.52±0.89. The frequency of allele values ranged from 0.0303 to 0.9333. The polymorphic information content (PIC) value ranged from 0.1227 to 0.7281. The overall means for observed (HO) and expected (He) heterozygosities were 0.545±0.279 and 0.548±0.179 respectively. The within-population inbreeding estimate (FIS) indicated heterozygosity deficiency of 0.0311. The Hardy-Weinberg equilibrium test revealed that 15 loci out of 21 deviated from equilibrium. The Shannon’s information index (I) was sufficiently high with a mean of 1 and bottleneck analysis revealed that population is not undergone any recent reduction.Keywords
Meghalaya Local Pig (Niang Megha), Heterozygosity, Microsatellites, PICFull Text
References
- Sambrook J, Fristisch E F et al. (1989). Molecular Cloning: a Laboratory Manual, 2nd Edn., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA.
- Henegariu O, Heerema N A et al. (1997). Multiplex PCR: critical parameters and step-by-step protocol, Biotechniques, vol 23(3), 504–511.
- Loffert D, Karger S et al. (1999). Optimization of multiplex PCR, Qiagen News, Issue 2, 5–8.
- Yeh F C, Boyle T et al. (1999). Popgene. Version 1.31. A Microsoft Windows based freeware for population genetic analysis, University of Alberta, Edmonton.
- Nei M (1978). Estimation of average heterozygosity and genetic distance from a small number of individuals, Genetics, vol 89, No. 3, 583–590.
- Cornuet J M, and Luikart G (1996). Description and power analysis of two tests for detecting recent population bottlenecks from allele frequency data, Genetics, vol 144, No. 4, 2001–2014.
- Food and Agriculture Organization (1998). In Guidelines for development of national farm animal genetic resources management plans, Measurement of Domestic Animal Diversity (MoDAD) recommended microsatellite markers, Food and Agriculture Organization of the United Nations, Rome, 19–24.
- Kaul R, Singh R K A et al. (2001). Evaluation of the genetic variability of 13 microsatellite markers in native Indian pigs, Journal of Genetics, vol 80(3), 149–153.
- Sollero B P, Paiva S R et al. (2010). Genetic diversity of Brazilian pig breeds evidenced by microsatellite markers, Livestock Science, vol 123(1), 8–15, DOI: 10.1016/j.livsci.2008.09.025.
- Swart H, Kortze A et al. (2010). Microsatellite-based characterization of Southern African domestic pigs (Sus scrofa domestica), South African Journal of Animal Science, vol 40(2), 121–132.
- Vicente A A, Carolino M I et al. (2008). Genetic diversity in native and commercial breeds of pigs in Portugal assessed by microsatellites, Journal of Animal Science, vol 86, No. 10, 2496–2507.
- Silva E P, and Russo C A M (2000). Techniques and statistical data analysis on molecular population genetics, Hydrobiologia, vol 420(1), 119–135.
- Genetic Integrity in Wild Stock of Babylonia spirata (Linnaeus, 1758) and Babylonia zeylanica (Bruguiere, 1789) from Southeast Coast of India: An important Mariculture Gastropod Species
Abstract Views :144 |
PDF Views:0
Authors
Affiliations
1 Centre of Advanced Study in Marine Biology, Annamalai University, Parangipettai-608502, Tamil Nadu, IN
2 Department of Biosciences, Saurashtra University, Rajkot-360005, Gujarat, IN
3 Department of Animal Genetics & Breeding, Veterinary College, Anand Agricultural University, Anand-388001, IN
1 Centre of Advanced Study in Marine Biology, Annamalai University, Parangipettai-608502, Tamil Nadu, IN
2 Department of Biosciences, Saurashtra University, Rajkot-360005, Gujarat, IN
3 Department of Animal Genetics & Breeding, Veterinary College, Anand Agricultural University, Anand-388001, IN
Source
Indian Journal of Science and Technology, Vol 9, No 4 (2016), Pagination:Abstract
Background/Objectives: Determining the effect of high demand of whelk meat, overexploitation and indiscriminate fishing on the genetic diversity in two economically important whelk species viz., Babylonia spirata and Babylonia zeylanica from southeast coast of India. Methods/Statistical analysis: The genetic diversity and population structure of two whelk species namely, B. spirata (62) and B. zeylanica (57) involving total 119 individuals were studied using standard diversity parameters. Both species were genotyped at 12 microsatellite loci to support conservation and improvement strategies. Findings: The results show that levels of genetic diversity in natural populations of specific genetic group are moderate to low. All the loci under study were observed to be highly polymorphic and a total of 139 alleles for all 12 markers were identified. The two genetic groups of the whelk species presented HWE deviations for majority of the loci. The range of alleles was found to be 3.5 to 7.5 with a global mean of 5.792. The overall mean of observed and expected heterozygosity were 0.489 and 0.787 respectively. Within population, inbreeding estimate (FIS = 0.381) indicated shortfall of heterozygosity in the population. Microsatellite analysis revealed less genetic diversity in both the species. The Analysis of Molecular Variance (AMOVA) showed 23% of total variation between both the species. Applications/Improvements: With the actual genetic diversity and the population structure of these two whelk genetic groups evaluated, it was possible to clarify their importance as well as to propose some management strategies to avoid further loss of genetic diversity in these whelk species.Keywords
Gastropod, Hardy-Weinberg Equilibrium, Heterozygosity, Polymorphic Information Content, Shannon Information IndexFull Text